Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179187" target="_blank" >RIV/00027006:_____/25:10179187 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15640/25:73632063

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S2667064X25003148/pdfft?md5=2381cc0a74dbf2ccd7a722a1c567cc3b&pid=1-s2.0-S2667064X25003148-main.pdf" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2667064X25003148/pdfft?md5=2381cc0a74dbf2ccd7a722a1c567cc3b&pid=1-s2.0-S2667064X25003148-main.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.stress.2025.101046" target="_blank" >10.1016/j.stress.2025.101046</a>

Result language

angličtina

Original language name

Polyamine uptake transporter 2 is essential for systemic acquired resistance establishment in Arabidopsis

Original language description

The study of polyamine transport in plants has become increasingly important due to the central role of these amines in regulating growth, development, adaptation, and stress responses. This research focused on the Arabidopsis thaliana Polyamine Uptake Transporters gene family under conditions of systemic acquired resistance. We evaluated all single mutants of this gene family and found that the put2-1 mutant abolished systemic acquired resistance while enhancing basal resistance to Pseudomonas syringae pv. tomato DC3000. In contrast, the 35S:: PUT2 overexpression lines showed improved resistance and reduced bacterial titers compared to wild-type plants. RNA-seq analysis revealed that the put2-1 mutant had deregulated expression of genes involved in the biosynthesis, signaling, and inactivation of salicylic acid and N-hydroxypipecolic acid. Most of these genes were transcriptionally upregulated by putrescine in wild-type plants, but not in the put2-1 mutant. Putrescine supplementation increased endogenous putrescine and salicylic acid levels in wild-type plants but not in put2-1, highlighting the essential role of this transporter in facilitating putrescine mobilization and regulating salicylic acid in distal tissues. We found that the defective systemic acquired resistance phenotype in the put2-1 mutant was linked to changes in the timing of polyamines, ROS, phenolic compound accumulation, and alterations in stomatal immunity. Our study emphasizes the key role of the Polyamine Uptake Transporter 2 (PUT2/LAT4) in establishing systemic acquired resistance in Arabidopsis, while also maintaining the plant&apos;s intrinsic basal resistance mechanisms. These findings offer valuable insights into the complex mechanisms of plant resistance, positioning polyamine transport as a central hub in systemic responses.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/EH23_021%2F0008909" target="_blank" >EH23_021/0008909: Interdisciplinary approaches for the development and application of new materials in industrial, agricultural and medical practice</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLANT STRESS

ISSN

2667-064X

e-ISSN

2667-064X

Volume of the periodical

18

Issue of the periodical within the volume

DEC 2025

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

19

Pages from-to

101046

UT code for WoS article

001588250800001

EID of the result in the Scopus database

2-s2.0-105017459523