Alginate Hydrogel with Pluronic F-68 Enhances Cryopreservation Efficiency in Peach Germplasm

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F25%3A10179352" target="_blank" >RIV/00027006:_____/25:10179352 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2310-2861/11/12/947/pdf" target="_blank" >https://www.mdpi.com/2310-2861/11/12/947/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/gels11120947" target="_blank" >10.3390/gels11120947</a>

Result language

angličtina

Original language name

Alginate Hydrogel with Pluronic F-68 Enhances Cryopreservation Efficiency in Peach Germplasm

Original language description

The long-term conservation of Prunus persica (peach), a crop of significant agronomic and genetic value, remains challenging due to its recalcitrance to conventional cryopreservation methods. Low tolerance to dehydration and cryoprotectant toxicity often results in poor survival and regrowth, thereby limiting the reliability of germplasm storage. This study evaluated whether combining an alginate hydrogel matrix with Pluronic F-68 improves vitrification efficiency and post-thaw regeneration of peach shoot tips by enhancing dehydration dynamics and reducing cryo-injury. Shoot tips were immobilized in thin sodium alginate layers on aluminum foil strips, with the hydrogel providing mechanical stabilization and moderating water loss during exposure to PVS3 and subsequent liquid nitrogen immersion. To further mitigate cryoinjury, Pluronic F-68, a non-ionic surfactant with membrane-stabilizing properties, was incorporated into the system. Differential scanning calorimetry revealed that the hydrogel reached complete vitrification after 120 min in PVS3, whereas encapsulated shoot tips required 150 min for full suppression of crystallization. The optimized system achieved 71% post-cryopreservation survival and 40% regrowth, compared with 25% and 9% in non-encapsulated controls. PF-68 accelerated vitrification kinetics, lowered crystallization enthalpies, and improved post-thaw viability. These findings demonstrate that engineered hydrogel–surfactant matrices can stabilize the microenvironment during vitrification and offer a promising approach for the long-term cryopreservation of peach germplasm.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

GELS

ISSN

2310-2861

e-ISSN

2310-2861

Volume of the periodical

11

Issue of the periodical within the volume

12

Country of publishing house

CH - SWITZERLAND

Number of pages

18

Pages from-to

947

UT code for WoS article

001646605600001

EID of the result in the Scopus database

2-s2.0-105026078157