Droplet-vitrification for shoot tip cryopreservation of shallot (Allium cepa var. aggregatum): effects of PVS3 and PVS2 on shoot regrowth

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F20%3A10145340" target="_blank" >RIV/00027006:_____/20:10145340 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs11240-019-01721-4" target="_blank" >https://link.springer.com/article/10.1007%2Fs11240-019-01721-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-019-01721-4" target="_blank" >10.1007/s11240-019-01721-4</a>

Result language

angličtina

Original language name

Droplet-vitrification for shoot tip cryopreservation of shallot (Allium cepa var. aggregatum): effects of PVS3 and PVS2 on shoot regrowth

Original language description

The present study described a droplet-vitrification cryopreservation for shoot tips of shallot (Allium cepa var. aggregatum), a small bulb onion. Shoot tips taken from in vitro stock shoots were precultured with 0.3 M and 0.5 M of sucrose, with 1 day for each concentration. Precultured shoot tips were treated with a loading solution containing 2 M glycerol and 0.6 M sucrose for 20 min and then exposed to plant vitrification solution 3 (PVS3) at 24 degrees C for 3 h of dehydration. Following exposure to PVS3, shoot tips were moved onto 5.0 mu l PVS3 droplets on aluminum foil strips, followed by direct immersion into liquid nitrogen for 1 h. Frozen shoot tips were thawed by incubation in liquid MS medium containing 1.2 m sucrose for 20 min at room temperature, and then post-thaw cultured for shoot regrowth. Exposure of the shoot tips to PVS3 produced shoot regrowth (58%). Differential scanning calorimetry (DSC) detected 1.8% of freezable water in the shoot tips that had been dehydrated by PVS2, and no freezable water in those by PVS3 treatment. Exposure to PVS3 provided a broader safe temperature range (- 196 degrees C to - 88 degrees C), compared to that (- 196 degrees C to - 116 degrees C) of PVS2, for cryopreserved samples. Histological observations found that PVS3 dehydration allowed many cells in the apical dome and in the leaf primordia to survive following freezing in LN, while PVS2 dehydration resulted in much fewer surviving cells in the apical dome. The droplet-vitrification cryopreservation produced 56%, 72% and 32% shoot regrowth in cryopreserved shoot tips taken from in vitro shoots, adventitious buds regenerated from stem discs and field-grown bulbs, respectively. The droplet-vitrification cryopreservation produced 45% and 70% shoot regrowth in the additional two shallot genotypes &apos;Kverve&apos; and &apos;Lunteviga&apos;. The results obtained in this study provide technical supports for setting-up cryo-bankings of genetic resources of shallots and other Allium species.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/QK1910476" target="_blank" >QK1910476: Garlic yield and quality increasing by selection drought and cold resistant clones based on molecular genetic analysis</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell, Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

—

Volume of the periodical

140

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

11

Pages from-to

185-195

UT code for WoS article

000517824500001

EID of the result in the Scopus database

2-s2.0-85074694695