Dehydration status of ABA-treated and cold-acclimated Hypericum perforatum L. shoot tips subjected to cryopreservation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F11%3A00001873" target="_blank" >RIV/00027006:_____/11:00001873 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.tca.2011.07.022" target="_blank" >http://dx.doi.org/10.1016/j.tca.2011.07.022</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tca.2011.07.022" target="_blank" >10.1016/j.tca.2011.07.022</a>
Alternative languages
Result language
angličtina
Original language name
Dehydration status of ABA-treated and cold-acclimated Hypericum perforatum L. shoot tips subjected to cryopreservation
Original language description
To improve the vitrification protocol for Hypericum perforatum shoot tips, calorimetric measurements of the intensity of thermal transitions in the samples during warming using differential scanning calorimetry were assessed. Comparison of ABA treatmentand cold-acclimation revealed higher content of crystallizable water in the latter. Regeneration capacity of cryopreserved H. perforatum shoot tips increased progressively with the exposure time in PVS3 cryoprotection mixture reaching the maximum between59.3 and 71.3% after 180 min when the proportion of freezable water ranged between 0.0 and 0.4% of total water content respectively. Glass transitions were observed in all samples dehydrated with PVS3 when freezable water was present. However, the decreasing recovery of H. perforatum shoot tips after prolonged PVS3 dehydration (without LN storage) indicated some injuries of meristematic tissue induced by PVS3 dehydration rather than ice nucleation and resulted from genotype-dependent to
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Thermochimica Acta
ISSN
0040-6031
e-ISSN
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Volume of the periodical
525
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
62-70
UT code for WoS article
000296824400009
EID of the result in the Scopus database
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