Application of differential thermal analysis for the improved regeneration after cryopreservation of vegetatively-propagated plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F19%3A00005116" target="_blank" >RIV/00027006:_____/19:00005116 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.7" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1234.7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1234.7" target="_blank" >10.17660/ActaHortic.2019.1234.7</a>

Result language

angličtina

Original language name

Application of differential thermal analysis for the improved regeneration after cryopreservation of vegetatively-propagated plants

Original language description

Three critical key parameters for cryoprotocol improvement resulting in increasing the regeneration rate after cryopreservation can be determined by thermal analysis: 1) the content of frozen water. The boundary content of frozen water affected by dehydration over desiccants or a mixture of cryoprotectants can be determined by differential scanning calorimetry; in the case of cryopreservation of garlic shoot tips, by dehydration in different concentrations of Plant Vitrification Solution No 3 (PVS3). Particular concentrations of cryoprotectants can regulate the final frozen water content. Although the regeneration of garlic shoot tips after their exposure to diluted PVS3 (i.e., sucrose/glycerol components 45/45) was similar to PVS3 (sucrose/glycerol 50/50), the use of less concentrated PVS3 reduces the risk of dehydration damage before ultra-low temperature application; 2) the content of unfreezable water. The intersection of the content of frozen water and the absolute content of water can be determined gravimetrically in shoot tips at different levels of dehydration and the content of unfreezable water derived. The unfreezable water content can be the limit of the minimum water content below which the shoot tips cannot survive dehydration; and 3) glass transition temperature. Determination of the glass transition temperature is important for long-term cryopreservation without undesirable alterations to the sample. A pure cryoprotectant, added usually in an excessive amount, has a constant Tg; whereas, the Tg of shoot tips increases as the content of water removed with the cryoprotective solution decreases. The highest recovery level after cryopreservation treatment should be achieved at the highest obtained sample glass transition temperature. The three above-mentioned parameters define the threshold dehydration level of shoot tips important for a high regeneration rate after cryopreservation.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

<a href="/en/project/QJ1630301" target="_blank" >QJ1630301: Development of the new biotechnological systems for maintanance and improvment of agriculture crop and forest tree biodiversitsy</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the III International Symposium on Plant Cryopreservation

ISBN

978-94-62612-31-0

ISSN

0567-7572

e-ISSN

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Number of pages

8

Pages from-to

57-64

Publisher name

International Society for Horticultural Science (ISHS)

Place of publication

Belgium

Event location

Bangkok, Thailand

Event date

Jan 1, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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