Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F11%3A00002048" target="_blank" >RIV/00027006:_____/11:00002048 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41610/11:53903

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Preparation of Shoot Tips by Sucrose and Dehydration Pre-Treatment of Ullucus tuberosus Cal. for Cryopreservation

Original language description

Ullucus tuberosus (ulluco) is a vegetatively propagated tuber crop of the Andean region. An method for cryopreservation of ulluco was tested with the aim of long term storage of U. tuberosus genetic resources. The effect of a sucrose pre-treatment improved the tolerance of shoot tips towards PVS3 dehydration. After two hours of desiccation over silicagel, the regeneration of thawed shoot tips from liquid nitrogen reached only 10%. This desiccation decreased the ice nucleation (taking place at -36°C) by6°C which could help to narrow down the time that lethal ice nucleation can take place before glass formation. PVS3 pre-treatment decreases the percentage of crystallizable water to less than 2% of water. The combination of sucrose pre-treatment and 2 hdesiccation over silica gel allowed 10% of the shoot tips to survive and regrow following cryopreservation. In the case where shoot tips were treated with PVS3 for 1.5 h, regeneration percentage reached 52.5%.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Volume of the periodical

neuveden

Issue of the periodical within the volume

908

Country of publishing house

BE - BELGIUM

Number of pages

8

Pages from-to

331-338

UT code for WoS article

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EID of the result in the Scopus database

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