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Droplet-vitrification methods for apical bud cryopreservation of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Rob.]

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F21%3A10174584" target="_blank" >RIV/00027006:_____/21:10174584 - isvavai.cz</a>

  • Alternative codes found

    RIV/60460709:41340/21:89229

  • Result on the web

    <a href="https://link.springer.com/journal/11240/volumes-and-issues/147-2" target="_blank" >https://link.springer.com/journal/11240/volumes-and-issues/147-2</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11240-021-02116-0" target="_blank" >10.1007/s11240-021-02116-0</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Droplet-vitrification methods for apical bud cryopreservation of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Rob.]

  • Original language description

    This study aimed to develop a cryopreservation protocol for the long-term preservation of yacon [Smallanthus sonchifolius (Poepp. and Endl.)], an Andean crop with high fructooligosaccharide content in its tuberous roots. Initially, the cryopreservation protocol was developed using a yacon clone originated from Ecuador classified as ECU 41. Osmotic dehydration of apical buds (2-3 mm long) was carried out by assessing two plant vitrification solutions, PVS2 (15, 30, and 60 min) at 0 degrees C and PVS3 (30, 45, 60, and 75 min) at 22 degrees C. After cryopreservation, the apical buds were thawed and placed on MS medium +/- 0.1 mg l(-1) N-6-benzyladenine (BA). The survival rates ranged from 37 to 90% within all treatments, with those subjected to PVS2 and PVS3 for 60 min showing the highest survival rates on MS medium without BA (87 and 90%, respectively). At 12 weeks post cryopreservation, these treatments also provided the highest regrowth rates, both reaching 73% of normally growing (shooting, rooting) plantlets. Survival rates on MS + 0.1 mg l(-1) BA regrowth medium reached up to 90%; however, regrowth into normally rooted plantlets did not exceed 67% post cryopreservation. The optimized protocols were then applied to 4 additional yacon clones originated from Bolivia and Peru, classified as BOL 22, BOL 23, PER 12, and PER 14. This resulted in survival and regeneration rates ranging between 79.7-94.1% and 66.3-75.4% respectively. Our study shows that optimal cryopreservation protocols for the long-term conservation of yacon can be based on both PVS2 and PVS3 vitrification solutions.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    PLANT CELL TISSUE AND ORGAN CULTURE

  • ISSN

    0167-6857

  • e-ISSN

  • Volume of the periodical

    147

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    12

  • Pages from-to

    197-208

  • UT code for WoS article

    000659829000001

  • EID of the result in the Scopus database

    2-s2.0-85107774349