Cryopreservation of strawberry (Fragaria x ananassa) germplasm and two aphid-transmitted strawberry viruses in Norway
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F24%3A00601756" target="_blank" >RIV/60077344:_____/24:00601756 - isvavai.cz</a>
Result on the web
<a href="https://www.pubhort.org/ejhs/89/3/15/index.htm" target="_blank" >https://www.pubhort.org/ejhs/89/3/15/index.htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/eJHS.2024/015" target="_blank" >10.17660/eJHS.2024/015</a>
Alternative languages
Result language
angličtina
Original language name
Cryopreservation of strawberry (Fragaria x ananassa) germplasm and two aphid-transmitted strawberry viruses in Norway
Original language description
Background of the study- Cryopreservation is considered to be a valuable method for long-term preservation of plant germplasm and recently it has been shown to be a reliable method for preserving obligate pathogens including plant viruses. Objectives- (1) Droplet-vitrification cryopreservation of strawberry genotypes in Norway, (2) Preservation efficiency of aphid-transmitted strawberry mild yellow edge virus (SMYEV) and strawberry vein banding virus (SVBV) following cryopreservation. Methods- Excised shoot tips of cv. Korona were cryopreserved with different durations of PVS2 varying from 10 to 60 min, whereas virus-infected shoot tips were cryopreserved using either 10, 40 or 60 min of PVS2. Results- The results showed that 40-60 minutes of PVS2 treatment was more efficient for preserving strawberry germplasm than lower duration times (10-30 min). Thirtytwo strawberry genotypes have been successfully cryopreserved through droplet-vitrification with regeneration rates ranging from 45% to 100% with 40 min PVS2 treatment. Cryopreserved viruses were quantitatively analyzed by Reverse Transcription-quantitative polymerase chain reaction (RT-qPCR). SVBV was successfully cryopreserved in all the regenerated shoots following cryopreservation with all the three durations of PVS2 examined. SMYEV, however, was more efficiently preserved in shoot tips exposed to 40 min (90%) of PVS2, in comparison to 60 min (33%). Conclusion-This demonstrates that SMYEV and SVBV can be successfully cryopreserved in living cells of Fragaria ssp. by droplet vitrification. The results indicate that cryopreservation has great potential for long-time preservation of both strawberry germplasm and aphid-transmitted strawberry-infecting viruses.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/TO01000295" target="_blank" >TO01000295: Healthy berries in a changing climate: development of new biotechnological procedures for virus diagnostics, vector studies, elimination and safe preservation of strawberry and raspberry</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Horticultural Scienc
ISSN
1611-4426
e-ISSN
1611-4434
Volume of the periodical
89
Issue of the periodical within the volume
3
Country of publishing house
BE - BELGIUM
Number of pages
8
Pages from-to
1-8
UT code for WoS article
001332714700001
EID of the result in the Scopus database
2-s2.0-85210445031