Erwinia amylovora - Biochemical Identification using the Biolog System

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027006%3A_____%2F99%3A%23%23%23%23%23899" target="_blank" >RIV/00027006:_____/99:#####899 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Erwinia amylovora - Biochemical Identification using the Biolog System

Original language description

The Biolog GN MicroPlate SystemTM was evaluated for accuracy in identifying of Erwinia amylovora, causing fire blight disease. Biolog Inc. (Hayward, CA, USA) MicroPlate system for diagnosis of gram-negative and gram-positive bacteria is based on different utilisation of series 95 carbon sources, including alcohols, polymeric chemicals, organic acids, organic acid salts and amino acids by individual taxonomic bacteria groups (Bochner 1989, Jones et al., 1993). All 95 carbon sources are placed in dried state in microplate. Microplate is inoculated by bacterial suspension and incubated for 4 and 24 hr at 28°C. Positive reaction utilisation of certain carbon source with tested bacteria is demonstrated by increased respiration, which is detected as resultant coloured reaction developed by reduction of tetrazolium viloet to a purple formazan. The resulting reaction pattern is called the bacterium's "metabolic fingerprint" (Jones et al., 1993).

Czech name

Ervinia amylovora - biochemická identifikace pomocí systému Biolog

Czech description

The Biolog GN MicroPlate SystemTM was evaluated for accuracy in identifying of Erwinia amylovora, causing fire blight disease. Biolog Inc. (Hayward, CA, USA) MicroPlate system for diagnosis of gram-negative and gram-positive bacteria is based on different utilisation of series 95 carbon sources, including alcohols, polymeric chemicals, organic acids, organic acid salts and amino acids by individual taxonomic bacteria groups (Bochner 1989, Jones et al., 1993). All 95 carbon sources are placed in dried state in microplate. Microplate is inoculated by bacterial suspension and incubated for 4 and 24 hr at 28°C. Positive reaction utilisation of certain carbon source with tested bacteria is demonstrated by increased respiration, which is detected as resultant coloured reaction developed by reduction of tetrazolium viloet to a purple formazan. The resulting reaction pattern is called the bacterium's "metabolic fingerprint" (Jones et al., 1993).

Type

D - Article in proceedings

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

1999

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Beiträge. 2. Symposium Phytomedizin und Pflanzenschutz im Gartenbau, Wien

ISBN

není přiděleno

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

170-171

Publisher name

Universität für Bodenkultur, Wien

Place of publication

Wien

Event location

Wien

Event date

Sep 27, 1999

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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