Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F01%3A00000097" target="_blank" >RIV/00027014:_____/01:00000097 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Effects of cell cycle dependent kinases inhibitor on nuclear and cytoplasmic maturation of porcine oocytes
Original language description
The aims of this study were to assess the effectiveness of roscovitine a potent inhibitor of cell cyclin kinases to prevent meiotic resumption in porcine oocytes and to test the subsequent fertilisability and developmental competence of these oocytes. Roscovitine blocked porcine oocytes at the GV stage during 22-44 hr of culture. This effect was dose-dependent and a concentration of 25 M was sufficient to prevent meiotic resumption in 92?5% of the oocytes after 22 hr in the presence of EGF and FSH.F andFSH. Cumulus expansion was also inhibited under these conditions. The histone H1 kinase activity in oocytes was inhibited in a dose-dependent way and maintained at a basal level with 25 M of roscovitine. Synthesis of proteins of 29 47 and 79 k normallysynthesized during maturation was inhibited too. All these effects were fully reversible.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Project
<a href="/en/project/GV524%2F96%2FK162" target="_blank" >GV524/96/K162: Cellular and molecular basis of oogenesis fertilization and embryonic development in mammals</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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