Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F09%3A%230001015" target="_blank" >RIV/00027014:_____/09:#0001015 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41210/09:31054

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor

Original language description

Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor (SNAP). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. Pulsatile treatments were more efficient with regard to the activation rate. The development was very limited after continuous treatment with SNAP. A significantly higher proportion of developing embryos was observed after the pulsatile treatment. However, this developmental rate was significantly lower than the development induced by treatment with calcium ionophore. After combination pulsatile treatment with the effect of protein kinase inhibitor 6-dimethyl aminopurine (6 DMAP) or with the inhibitor of protein synthesis cycloheximide (CHX) a significant increase in the activation rate when compared to the respective pulsatile treatment was observed. However, the development of parthenogenetic embryos was not enhanced when the pulsatile treatment was combined with 6-DMAP or with CHX..

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA523%2F06%2F0295" target="_blank" >GA523/06/0295: Activation of pig oocytes using nitric oxide donors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Czech Journal of Animal Science

ISSN

1212-1819

e-ISSN

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Volume of the periodical

54

Issue of the periodical within the volume

7

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

14

Pages from-to

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UT code for WoS article

000268207300001

EID of the result in the Scopus database

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