Parthenogenetic activation of pig oocytes using pulsatile treatment with a nitric oxide donor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F10%3A48294" target="_blank" >RIV/60460709:41210/10:48294 - isvavai.cz</a>

Alternative codes found

RIV/68378041:_____/10:00343568 RIV/00027014:_____/10:#0001204

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Parthenogenetic activation of pig oocytes using pulsatile treatment with a nitric oxide donor

Original language description

The nitric oxide donor (+)S-nitroso-N-acetylpenicillamine (SNAP) is capable of inducing parthenogenetic activation in pig oocytes. Repeated short-term treatment with 2 mM SNAP increased the activation rates. With regard to the activation rate, 12 repeated treatments lasting 10 min each were found to be the most efficient regimen (63.3%). The continuous exposure to the NO donor for the same overall time induced parthenogenetic activation in 12.5% oocytes (2-h) continuous treatment with 2 mM SNAP). The development of parthenogenetic embryos increased after repeated short-term treatment with SNAP. After continuous treatment with 2 mM SNAP for 10 h, only 6.7% of the oocytes cleaved, and none developed beyond the 4-cell stage. Thirty-minute treatment repeated four times with 2 mM SNAP induced cleavage in 37.5% of the oocytes, 18.3% developed to the morula stage, and 6.7% reached the blastocyst stage. It is concluded that pulsatile treatment can improve parthenogenetic activation rate.

Czech name

Parthenogenetic activation of pig oocytes using pulsatile treatment with a nitric oxide donor

Czech description

The nitric oxide donor (+)S-nitroso-N-acetylpenicillamine (SNAP) is capable of inducing parthenogenetic activation in pig oocytes. Repeated short-term treatment with 2 mM SNAP increased the activation rates. With regard to the activation rate, 12 repeated treatments lasting 10 min each were found to be the most efficient regimen (63.3%). The continuous exposure to the NO donor for the same overall time induced parthenogenetic activation in 12.5% oocytes (2-h) continuous treatment with 2 mM SNAP). The development of parthenogenetic embryos increased after repeated short-term treatment with SNAP. After continuous treatment with 2 mM SNAP for 10 h, only 6.7% of the oocytes cleaved, and none developed beyond the 4-cell stage. Thirty-minute treatment repeated four times with 2 mM SNAP induced cleavage in 37.5% of the oocytes, 18.3% developed to the morula stage, and 6.7% reached the blastocyst stage. It is concluded that pulsatile treatment can improve parthenogenetic activation rate.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Reproduction in Domestic Animals

ISSN

0936-6768

e-ISSN

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Volume of the periodical

45

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

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UT code for WoS article

000277873000019

EID of the result in the Scopus database

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