The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11691" target="_blank" >RIV/60460709:41210/05:11691 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The role of extracellular and intracellular calcium stores in NO-dependent parthenogenetic activation of pig oocytes

Original language description

Pig oocytes matured in vitro are parthenogenetically actived (70%) after the treatment with 2mM NO-donor SNAP for 10 hours. The chelation of intracellular calcium ions after the treatment with BAPTA-AM suppressed the SNAP-induced activation in dose-dependent manner (56% activated oocytes after the treatment with 5 mikroM BAPTA-AM; non activated oocyte after the treatment with 50 mikroM BAPTA-AM). Activation by NOdonor is dependent on the influx of calcium from extracellular spaces, because the blockageof calcium channels by verapamil significantly reduced the activation rate SNAP-treated oocytes (17% of activated oocytes after the treatment with 0,08 mM verapamil). These data indicate that the activation of pig oocytes with NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular stores.

Czech name

Úloha extracelárních a intracelulárních vápníkových depozit při NO-dependentní partenogenetické aktivaci prasečích oocytů

Czech description

Pig oocytes matured in vitro are parthenogenetically actived (70%) after the treatment with 2mM NO-donor SNAP for 10 hours. The chelation of intracellular calcium ions after the treatment with BAPTA-AM suppressed the SNAP-induced activation in dose-dependent manner (56% activated oocytes after the treatment with 5 mikroM BAPTA-AM; non activated oocyte after the treatment with 50 mikroM BAPTA-AM). Activation by NOdonor is dependent on the influx of calcium from extracellular spaces, because the blockageof calcium channels by verapamil significantly reduced the activation rate SNAP-treated oocytes (17% of activated oocytes after the treatment with 0,08 mM verapamil). These data indicate that the activation of pig oocytes with NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular stores.

Type

D - Article in proceedings

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Current Problems of Breeding, Health, Growth and Production Pigs, České Budějovice

ISBN

80-85645-50-5

ISSN

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e-ISSN

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Number of pages

4

Pages from-to

265-268

Publisher name

Biologická fakulta

Place of publication

České Budějovice

Event location

České Budějovice

Event date

Feb 9, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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