Nitric oxide-dependent activation of pig oocytes: role of calcium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F05%3A11363" target="_blank" >RIV/60460709:41210/05:11363 - isvavai.cz</a>

Alternative codes found

RIV/00027014:_____/05:#0000122

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Nitric oxide-dependent activation of pig oocytes: role of calcium

Original language description

Pig oocytes matured in vitro are parthenogenetically activated after treatment with nitric oxide (NO)-donor SNAP. The chelation of intracellular calcium ions withBAPTA-AM suppressed the SNAP-induced activation in a dose-dependent manner. Activation by aNO-donor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil had significantly reduced the activation rate in SNAP-treated oocytes. The blockage of inositol triphosphate receptors had noeffect on the activation of oocytes by a NO-donor. On the other hand, the blockers of ryanodine receptors, procaine and ruthenium red, inhibited the activation of oocytes induced by a NO-donor. These data indicate that the activation of pig oocytes by aNO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular spaces. For the mobilization of intracellular calcium stores, it is the ryanodine receptors and not the inositol triphosphate

Czech name

Aktivace oocytů prasete závislá na oxidu dusnatém: úloha vápníku

Czech description

Pig oocytes matured in vitro are parthenogenetically activated after treatment with nitric oxide (NO)-donor SNAP. The chelation of intracellular calcium ions withBAPTA-AM suppressed the SNAP-induced activation in a dose-dependent manner. Activation by aNO-donor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil had significantly reduced the activation rate in SNAP-treated oocytes. The blockage of inositol triphosphate receptors had noeffect on the activation of oocytes by a NO-donor. On the other hand, the blockers of ryanodine receptors, procaine and ruthenium red, inhibited the activation of oocytes induced by a NO-donor. These data indicate that the activation of pig oocytes by aNO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular spaces. For the mobilization of intracellular calcium stores, it is the ryanodine receptors and not the inositol triphosphate

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

<a href="/en/project/GD523%2F03%2FH076" target="_blank" >GD523/03/H076: Enhancing of Methodological Level and Theoretical Education of Students of the Ph. D. Program 4103V - Animal Husbandry - a Perspective Field General Animal Husb</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecullar and Cellular Endocrinology

ISSN

0303-7207

e-ISSN

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Volume of the periodical

242

Issue of the periodical within the volume

1

Country of publishing house

IE - IRELAND

Number of pages

7

Pages from-to

16-22

UT code for WoS article

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EID of the result in the Scopus database

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