Nitric-oxide-dependent activation of pig oocytes: the role of the cGMP - signalling pathway

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F06%3A16235" target="_blank" >RIV/60460709:41210/06:16235 - isvavai.cz</a>

Alternative codes found

RIV/00027014:_____/06:#0000264

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Nitric-oxide-dependent activation of pig oocytes: the role of the cGMP - signalling pathway

Original language description

Pig oocytes matured in vitro were parthenogenetically activated (78 %) after the treatment with 2 mM nitric oxide-donor (?)-S-nitroso-N-acetylpenicillamine (SNAP) for 24 hours. Inhibition of soluble guanylyl cyclase with specific inhibitors 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) or 6-anilino-5,8-quinolinequinone (LY83583) suppressed the SNAP-induced activation in a dose-dependent manner (23% of activated oocytes after the treatment with 400 ?M of ODQ; 12% of activated oocytes after treatmentwith 40 ?M of LY83583). 8- bromo-cyclic guanosine monophosphate (8-Br-cGMP), a phoshodiesterase resistant analog of cGMP, enhances the effect of suboptimal doses (0.1 or 0.5 mM) of the NO-donor SNAP. DT3 a specific inhibitor of cGMP-dependent protein kinase (protein kinase G - PKG) is also able to inhibit the activation of pig oocytes after NO-donor treatment. Involvement of the cGMP-dependent signaling pathway is specific for NO-induced oocyte activation, because both the guanylyl cycla

Czech name

NO dependentní aktivace prasečích oocytů: úloha cGMP dependentní signální kaskády

Czech description

Pig oocytes matured in vitro were parthenogenetically activated (78 %) after the treatment with 2 mM nitric oxide-donor (?)-S-nitroso-N-acetylpenicillamine (SNAP) for 24 hours. Inhibition of soluble guanylyl cyclase with specific inhibitors 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) or 6-anilino-5,8-quinolinequinone (LY83583) suppressed the SNAP-induced activation in a dose-dependent manner (23% of activated oocytes after the treatment with 400 ?M of ODQ; 12% of activated oocytes after treatmentwith 40 ?M of LY83583). 8- bromo-cyclic guanosine monophosphate (8-Br-cGMP), a phoshodiesterase resistant analog of cGMP, enhances the effect of suboptimal doses (0.1 or 0.5 mM) of the NO-donor SNAP. DT3 a specific inhibitor of cGMP-dependent protein kinase (protein kinase G - PKG) is also able to inhibit the activation of pig oocytes after NO-donor treatment. Involvement of the cGMP-dependent signaling pathway is specific for NO-induced oocyte activation, because both the guanylyl cycla

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GG - Zootechnics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Zygote

ISSN

0967-1994

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

14

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

9-16

UT code for WoS article

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EID of the result in the Scopus database

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