Optimization of Conditions of Prolonged Culture of Pig Granulosa Cells in Vitro

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F11%3A%230001442" target="_blank" >RIV/00027014:_____/11:#0001442 - isvavai.cz</a>
Result on the web
<a href="http://www.vuzv.cz/sites/File/_privat/11078.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/11078.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Optimization of Conditions of Prolonged Culture of Pig Granulosa Cells in Vitro
Original language description
The object of the present study was to establish conditions (optimal serum concentration and growth factor supplement) for prolonged culture of pig granulosa cells (GC). GC were cultured in the presence of different fetal calf serum (FCS) concentrations(10% and 20%) and one of the following growth factors: leukemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF) and stem cell factor (SCF). GC proliferation potential was measured by 3H-thymidine incorporation and telomerase activity whereas cell differentiation by relative transcript abundance of the aromatase gene. In the presence of 10% FCS GC proliferative potential was significantly higher (P<0.01) in comparison to control (medium only) after 9 days of culture. Basic fibroblast growth factor significantly increased (P<0.01) 3H-thymidine incorporation at all investigated time intervals/points, up to 18 days of culture. Telomerase activity was stimulated (P<0.01) by LIF, bFGF and SCF after 3, 6, 9, 12, 15 and 18 days o
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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