Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F12%3A%230001661" target="_blank" >RIV/00027014:_____/12:#0001661 - isvavai.cz</a>
Result on the web
<a href="http://www.vuzv.cz/sites/File/_privat/12104.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/12104.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells
Original language description
The aim of the present work was to determine the relative transcript abundance of the oestrogen receptor alpha (ER alpha) and beta (ER beta) gene in long-term cultures (up to 18 days) of pig granulosa cells (GC) obtained from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. GC were cultured in the presence of 10% foetal calf serum (FCS) and one of the following growth factors: leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF), or stem cell factor (SCF). The relative transcript abundance of the investigated genes was measured by real time PCR (RT-PCR). In comparison with the control (medium only), LIF significantly increased (P<0.05-0.01) ER alpha gene expression in SF-GC after 3, 15 and 18 days of culture and after 12 days in LF-GC. The ER beta mRNA level in SF-GC was significantly decreased (P<0.01) by all of the investigated factors after 3, 6, 9 and 12 days of incubation. In conclusion, expression of both types of oestrogen receptors during prolonged
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Animal and Feed Sciences
ISSN
1230-1388
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
2
Country of publishing house
PL - POLAND
Number of pages
11
Pages from-to
313-323
UT code for WoS article
000306726400008
EID of the result in the Scopus database
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