Technical note: A novel method for routine genotyping of the G allele of ?-casein (CSN2) and T allele of ?-casein (CSN3) in a sheep population using LightCycler
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F11%3A%230001491" target="_blank" >RIV/00027014:_____/11:#0001491 - isvavai.cz</a>
Result on the web
<a href="http://www.vuzv.cz/sites/File/_privat/11128.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/11128.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2527/jas.2011-4054" target="_blank" >10.2527/jas.2011-4054</a>
Alternative languages
Result language
angličtina
Original language name
Technical note: A novel method for routine genotyping of the G allele of ?-casein (CSN2) and T allele of ?-casein (CSN3) in a sheep population using LightCycler
Original language description
The goal of this work was to develop a protocol for rapid genotyping of A and G variants at the CSN2 locus and genotyping of T and C variants at the CSN3 locus in sheep breeds (Sumava and Valachian) by means of PCR and LightCycler analysis. The LightCycler technique combines rapid and efficient in vitro amplification of DNA in glass capillaries with melting curve analysis based on fluorescence resonance energy transfer for the sensitive detection of point mutation. The A variant had a greater frequency(Sumava, 0.778; Valachian, 0.835) than did variant G (Sumava, 0.222; Valachian, 0.165) in both sheep breeds. The CSN3 locus was found to be monomorphic, with no polymorphism identified in either population.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Animal Science
ISSN
0021-8812
e-ISSN
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Volume of the periodical
89
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
3
Pages from-to
3843-3845
UT code for WoS article
000297368200001
EID of the result in the Scopus database
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