Simultaneous genotyping of 4 SNPs in promoter III of the ovine ACACA

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F16%3AN0000029" target="_blank" >RIV/00027014:_____/16:N0000029 - isvavai.cz</a>
Result on the web
<a href="http://www.vuzv.cz/sites/File/_privat/16028.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/16028.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.smallrumres.2016.03.027" target="_blank" >10.1016/j.smallrumres.2016.03.027</a>

Result language
angličtina
Original language name
Simultaneous genotyping of 4 SNPs in promoter III of the ovine ACACA
Original language description
biosynthesis of fatty acids in animal tissues. By sequencing analysis in promoter III of the ovine ACACA we identified 2 novel SNPs (A > G at 1168 bp and a T nucleotide insertion at 1182 bp) (GenBank accession number AJ292286). The novel variant (A > G at 1168 bp) was present in Cigaja, Sumavka, and Improved Valachian breed kept in the Czech Republic and T insertion at 1182 bp was present in the East Friesian breed. A protocol for rapid efficient simultaneous genotyping of ACACA variants using the primer extension method (PEA) was described, and a total of 171 animals belonging to sheep breeds: Cigaja (9), Sumavka (87), East Friesian (59) and Improved Valachian (16) were genotyped. Variant alleles are: A/G at 1168 bp, G/T at 1330 bp, C/G at 1138 bp and C/T at 1430 bp.The genotype combination AG/GT/CG/CC occurred in all 4 breeds kept in the Czech Republic with frequency of occurrence ranging from 15.25% to 44.45%. Allele frequencies of the Cigaja breed differ significantly from the other Czech sheep breeds
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/QJ1310107" target="_blank" >QJ1310107: Effect genetic polymorphism lipogenic enzymes on the milk fat,composition of the fatty acids in milk in small rumimants (goat, sheep).</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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