Role of Choline and Methionine in Bovine Mammary Epithelial Cell Line Exposed to Hydrogen Peroxide
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F13%3A%230001941" target="_blank" >RIV/00027014:_____/13:#0001941 - isvavai.cz</a>
Result on the web
<a href="http://www.vuzv.cz/sites/File/_privat/13176.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/13176.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1166/jnef.2013.1033" target="_blank" >10.1166/jnef.2013.1033</a>
Alternative languages
Result language
angličtina
Original language name
Role of Choline and Methionine in Bovine Mammary Epithelial Cell Line Exposed to Hydrogen Peroxide
Original language description
Choline and methionine are essential nutrients for many mammals. In this work we aimed to evaluate 1^ role of choline and methionine in counteracting the oxidative stress induced by hydrogen perowein bovine mammary epithelial cells. BME-UV1 cell line hasbeen previously used as in vitro model for mammary epithelium. First of all, BME-UV1 were exposed to increasing concentrations of hydrogen peroxide to establish half lethal concentrations at 24, 48 and 72 hours. Furthermore, BME-UV1 cells were incubatedwith increasing amounts of choline and methionine in order to identify the most effective concentrations in term of cell viability. In this respect, two combinations of choline and methionine have been selected: a Low Choline/Methionine dosage and a High Choline/Methionine dosage, providing respectively to 500 yu.M/715 ytiM and 1000 ytiM/1430 ytiM of choline and methionine. After that, BME-UV1 treated with Low Choline/Methionine dosage and High Choline/Methionine dosage were exposed to
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Nutritional Ecology and Food Research
ISSN
2326-4225
e-ISSN
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Volume of the periodical
1
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
189-193
UT code for WoS article
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EID of the result in the Scopus database
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