Multilocus sequence typing of cronobacter strains isolated from retail foods and environmental samples  

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F15%3A%230002299" target="_blank" >RIV/00027014:_____/15:#0002299 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/15:00456506 RIV/60460709:41210/15:67575 RIV/60461373:22330/15:43900116

Result on the web

<a href="http://www.vuzv.cz/sites/File/_privat/15150.pdf" target="_blank" >http://www.vuzv.cz/sites/File/_privat/15150.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/fpd.2014.1884" target="_blank" >10.1089/fpd.2014.1884</a>

Result language

angličtina

Original language name

Multilocus sequence typing of cronobacter strains isolated from retail foods and environmental samples  

Original language description

Cronobacter spp. are bacterial pathogens that affect children and immunocompromised adults. In this study, we used multilocus sequence typing (MLST) to determine sequence types (STs) in 11 Cronobacter spp. strains isolated from retail foods, 29 strains from dust samples obtained from vacuum cleaners, and 4 clinical isolates. Using biochemical tests, species-specific polymerase chain reaction, and MLST analysis, 36 strains were identified as Cronobacter sakazakii, and 6 were identified as Cronobacter malonaticus. In addition, one strain that originated from retail food and one from a dust sample from a vacuum cleaner were identified on the basis of MLST analysis as Cronobacter dublinensis and Cronobacter turicensis, respectively. Cronobacter spp. strains isolated from the retail foods were assigned to eight different MLST sequence types, seven of which were newly identified. The strains isolated from the dust samples were assigned to 7 known STs and 14 unknown STs. Three clinical isolates and one household dust isolate were assigned to ST4, which is the predominant ST associated with neonatal meningitis. One clinical isolate was classified based on MLST analysis as Cronobacter malonaticus and belonged to an as-yet-unknown ST. Three strains isolated from the household dust samples were assigned to ST1, which is another clinically significant ST. It can be concluded that Cronobacter spp. strains of different origin are genetically quite variable. The recovery of C. sakazakii strains belonging to ST1 and ST4 from the dust samples suggests the possibility that contamination could occur during food preparation. All of the novel STs and alleles for C. sakazakii, C. malonaticus, C. dublinensis, and C. turicensis determined in this study were deposited in the Cronobacter MLST database available online (http://pubmlst.org/cronobacter/).

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Foodborne Pathogens and Disease 

ISSN

1535-3141

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

514-521

UT code for WoS article

000368681900007

EID of the result in the Scopus database

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