Characterization of Cronobacter spp. isolated from food of plant origin and environmental samples collected on farms and from supermarkets in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F16%3AN0000021" target="_blank" >RIV/00027162:_____/16:N0000021 - isvavai.cz</a>

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0168160515301501" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0168160515301501</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijfoodmicro.2015.10.017" target="_blank" >10.1016/j.ijfoodmicro.2015.10.017</a>

Result language

angličtina

Original language name

Characterization of Cronobacter spp. isolated from food of plant origin and environmental samples collected on farms and from supermarkets in the Czech Republic

Original language description

The Cronobacter genus (previously known as Enterobacter sakazakii) comprises seven species (C. sakazakii, C. malonaticus, C. muytjensii, C. turicensis, C. dublinensis, C. universalis and C. condimenti) which cause serious infections in neonates and immunocompromised people. Most of the documented outbreaks of these bacteria have been associated with consumption of contaminated powdered infant formula. The plant environment is considered to be the natural habitat of these bacteria. Therefore, a total number of 563 samples of vegetables, fruit, water and environmental swabs were collected from local farms and supermarkets in the Czech Republic and investigated for the presence of Cronobacter spp. The obtained 45 isolates (8.0%) were further characterized by phenotyping (antimicrobial resistance, capsule and pigment production) and genotyping (fusA sequencing, MLST, PCR-serotyping) methods. Most of the Cronobacter isolates (42.2%) were identified as C. sakazakii, followed by C. turicensis (31.1%), C. dublinensis (22.2%), C. malonaticus (2.2%) and C. universalis (2.2%). The 25 identified sequence types, out of which 17 were unique for only one strain, indicated a high diversity of strains. C. sakazakii sequence type 4 (ST 4), which has been associated with many cases of meningitis, was isolated only in one case. A strong association of C. turicensis and C. dublinensis with the plant environment can be deduced from our results.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/LO1218" target="_blank" >LO1218: Healthy animal as a source of wholesome food</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Food Microbiology

ISSN

0168-1605

e-ISSN

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Volume of the periodical

217

Issue of the periodical within the volume

JAN

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

130-136

UT code for WoS article

000366767600018

EID of the result in the Scopus database

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