Dissecting the role of the germinal vesicle nuclear envelope and soluble
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F19%3AN0000175" target="_blank" >RIV/00027014:_____/19:N0000175 - isvavai.cz</a>
Result on the web
<a href="https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-017/_pdf" target="_blank" >https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-017/_pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1262/jrd.2019-017" target="_blank" >10.1262/jrd.2019-017</a>
Alternative languages
Result language
angličtina
Original language name
Dissecting the role of the germinal vesicle nuclear envelope and soluble
Original language description
Differentiated nuclei can be reprogrammed/remodelled to totipotency after their transfer to enucleated metaphase II (MII) oocytes. The process of eprogramming/remodelling is, however, only partially characterized. It has been shown that the oocyte nucleus (germinal vesicle – GV) components are essential for a successful remodelling of the transferred nukleus by providing the materials for pseudo-nucleus formation. However, the nucleus is a complex structure and exactly what nuclear components are required for a successful nucleus remodelling and reprogramming is unknown. Till date, the only nuclear sub-structure experimentally demonstrated to be essential is the oocyte nucleolus (nucleolus-like body, NLB). In this study, we investigated what other GV components might be necessary for the formation of normal-sized pseudo-pronuclei (PNs). Our results showed that the removal of the GV nuclear envelope with attached chromatin and chromatin-bound factors does not substantially influence the size of the remodelled nuclei in reconstructed cells and that their nuclear envelopes seem to have normal parameters. Rather than the insoluble nuclear lamina, the GV content, which is dissolved in the cytoplasm with the onset of oocyte maturation, influences the characteristics and size of transferred nuclei.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
<a href="/en/project/GA17-08605S" target="_blank" >GA17-08605S: Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation and embryo development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Reproduction and Development
ISSN
0916-8818
e-ISSN
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Volume of the periodical
65
Issue of the periodical within the volume
5
Country of publishing house
JP - JAPAN
Number of pages
9
Pages from-to
433-441
UT code for WoS article
000494886200006
EID of the result in the Scopus database
2-s2.0-85074117825