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Dissecting the role of the germinal vesicle nuclear envelope and soluble

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F19%3AN0000175" target="_blank" >RIV/00027014:_____/19:N0000175 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-017/_pdf" target="_blank" >https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-017/_pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1262/jrd.2019-017" target="_blank" >10.1262/jrd.2019-017</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Dissecting the role of the germinal vesicle nuclear envelope and soluble

  • Original language description

    Differentiated nuclei can be reprogrammed/remodelled to totipotency after their transfer to enucleated metaphase II (MII) oocytes. The process of eprogramming/remodelling is, however, only partially characterized. It has been shown that the oocyte nucleus (germinal vesicle – GV) components are essential for a successful remodelling of the transferred nukleus by providing the materials for pseudo-nucleus formation. However, the nucleus is a complex structure and exactly what nuclear components are required for a successful nucleus remodelling and reprogramming is unknown. Till date, the only nuclear sub-structure experimentally demonstrated to be essential is the oocyte nucleolus (nucleolus-like body, NLB). In this study, we investigated what other GV components might be necessary for the formation of normal-sized pseudo-pronuclei (PNs). Our results showed that the removal of the GV nuclear envelope with attached chromatin and chromatin-bound factors does not substantially influence the size of the remodelled nuclei in reconstructed cells and that their nuclear envelopes seem to have normal parameters. Rather than the insoluble nuclear lamina, the GV content, which is dissolved in the cytoplasm with the onset of oocyte maturation, influences the characteristics and size of transferred nuclei.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10604 - Reproductive biology (medical aspects to be 3)

Result continuities

  • Project

    <a href="/en/project/GA17-08605S" target="_blank" >GA17-08605S: Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation and embryo development</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Reproduction and Development

  • ISSN

    0916-8818

  • e-ISSN

  • Volume of the periodical

    65

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    JP - JAPAN

  • Number of pages

    9

  • Pages from-to

    433-441

  • UT code for WoS article

    000494886200006

  • EID of the result in the Scopus database

    2-s2.0-85074117825