Dissecting the role of the germinal vesicle nuclear envelope and soluble content in the process of somatic cell remodelling and reprogramming

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F19%3A00523655" target="_blank" >RIV/68378041:_____/19:00523655 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/19:00523643

Result on the web

<a href="https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-017/_article" target="_blank" >https://www.jstage.jst.go.jp/article/jrd/65/5/65_2019-017/_article</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1262/jrd.2019-017" target="_blank" >10.1262/jrd.2019-017</a>

Result language

angličtina

Original language name

Dissecting the role of the germinal vesicle nuclear envelope and soluble content in the process of somatic cell remodelling and reprogramming

Original language description

Differentiated nuclei can be reprogrammed/remodelled to totipotency after their transfer to enucleated metaphase II (MII) oocytes. The process of reprogramming/remodelling is, however, only partially characterized. It has been shown that the oocyte nucleus (germinal vesicle - GV) components are essential for a successful remodelling of the transferred nucleus by providing the materials for pseudo-nucleus formation. However, the nucleus is a complex structure and exactly what nuclear components are required for a successful nucleus remodelling and reprogramming is unknown. Till date, the only nuclear sub-structure experimentally demonstrated to be essential is the oocyte nucleolus (nucleolus-like body, NLB). In this study, we investigated what other GV components might be necessary for the formation of normal-sized pseudo-pronuclei (PNs). Our results showed that the removal of the GV nuclear envelope with attached chromatin and chromatin-bound factors does not substantially influence the size of the remodelled nuclei in reconstructed cells and that their nuclear envelopes seem to have normal parameters. Rather than the insoluble nuclear lamina, the GV content, which is dissolved in the cytoplasm with the onset of oocyte maturation, influences the characteristics and size of transferred nuclei.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Reproduction and Development

ISSN

0916-8818

e-ISSN

—

Volume of the periodical

65

Issue of the periodical within the volume

5

Country of publishing house

JP - JAPAN

Number of pages

9

Pages from-to

433-441

UT code for WoS article

000494886200006

EID of the result in the Scopus database

2-s2.0-85074117825