Assessing the effect of interspecies oocyte nucleolar material dosage on embryonic development
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F20%3AN0000066" target="_blank" >RIV/00027014:_____/20:N0000066 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/20:00533593 RIV/67985904:_____/20:00533593 RIV/00027014:_____/20:N0000065 RIV/00216208:11120/20:43920572
Result on the web
<a href="https://vuzv.cz/_privat/20063.pdf" target="_blank" >https://vuzv.cz/_privat/20063.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.theriogenology.2020.06.001" target="_blank" >10.1016/j.theriogenology.2020.06.001</a>
Alternative languages
Result language
angličtina
Original language name
Assessing the effect of interspecies oocyte nucleolar material dosage on embryonic development
Original language description
Sequence differences are considered to be the basic cause of developmental failure in interspecies embryos when more distant species are combined. However, other phenomena, such as insufficient or excessive quantity of specific cellular factors, might also influence the outcome. These effects are usually not considered. One of the organelles shown to contain different amount of proteins is the oocyte nucleolus-like body. Here we show that upon interspecies transfer, a single porcine nucleolus-like body is unable to support the development of a mouse parthenogenetic embryo derived from an enucleolated oocyte. However, when the amount of the porcine nucleolar material is increased to equalize the amount of mouse nucleolar material by transferring two nucleolus-like bodies, mouse embryos are able to pass the developmental block elicited by enucleolation. These embryos progress to the blastocyst stage at rates comparable to controls. Thus, using the model of an interspecies nucleolus-like body transplantation between mouse and pig oocytes, we show that an inadequate amount of nucleolar factors, rather than the species origin, affects the development. In a wider context of interspecies nuclear transfer schemes, the observed incompatibility between more distant species might not stem simply from sequence differences but also from improper dosage of key cellular factors.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10604 - Reproductive biology (medical aspects to be 3)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Theriogenology
ISSN
0093-691X
e-ISSN
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Volume of the periodical
155
Issue of the periodical within the volume
n
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
17-24
UT code for WoS article
000566760000003
EID of the result in the Scopus database
2-s2.0-85086702939