Mouse Nucleolus-Like Body Supports Transcription and Enhances Processing of rRna in Porcine Enucleolated Oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F25%3A00641200" target="_blank" >RIV/67985904:_____/25:00641200 - isvavai.cz</a>

Result on the web

<a href="https://reference-global.com/article/10.2478/macvetrev-2025-0011" target="_blank" >https://reference-global.com/article/10.2478/macvetrev-2025-0011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/macvetrev-2025-0011" target="_blank" >10.2478/macvetrev-2025-0011</a>

Result language

angličtina

Original language name

Mouse Nucleolus-Like Body Supports Transcription and Enhances Processing of rRna in Porcine Enucleolated Oocytes

Original language description

Fully-grown mammalian oocytes contain morphologically evident but transcriptionally inactive nucleoli called nucleolus-like bodies (NLBs). These nuclear structures are essential for early embryonic development. Removing oocyte NLBs (enucleolation) before their activation leads to developmental failure, mainly at the time point of embryonic genome activation. The present study examined the developmental and expression dynamics of embryos derived from intra- (pig) and interspecies (mouse) nucleolus (NLB) transferred porcine oocytes after parthenogenetic activation. Activation of rDNA transcription and pre-rRNA processing in NLB re-injected embryos was observed by real-time qRT-PCR analysis, targeting the expression rates of 45S rRNA and levels of 18S rRNA. Reinjection of NLBs from mouse or porcine GV oocytes into the enucleolated MII stage porcine oocytes supported the embryonic development up to the blastocyst stage after their activation. Intra- and intergeneric NLB transferred embryos demonstrated rDNA transcription initiation at the 8-cell stage, corresponding with the embryonic genome activation in porcine parthenogenetic embryos (control). The measured levels of 18S rRNA in these experimental embryos showed delayed initiation of pre-rRNA processing at the blastocysts stage compared with the control (8-cell stage). However, the porcine embryos with re-injected mouse NLB displayed significantly higher levels of 18S rRNA than the control and experimental intraspecies group. In conclusion, NLBs from different mammal species (mouse) can enhance the quality of enucleolated porcine oocytes and thus support their early embryonic development after activation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10605 - Developmental biology

Project

<a href="/en/project/EF15_003%2F0000460" target="_blank" >EF15_003/0000460: EXCELLENCE in Molecular Aspects of the early development of vertebrates</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Macedonian veterinary review

ISSN

1409-7621

e-ISSN

1857-7415

Volume of the periodical

48

Issue of the periodical within the volume

1

Country of publishing house

MK - REPUBLIC OF NORTH MACEDONIA

Number of pages

7

Pages from-to

15-21

UT code for WoS article

001413655500001

EID of the result in the Scopus database

2-s2.0-105001208195