Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F23%3A10005830" target="_blank" >RIV/00027014:_____/23:10005830 - isvavai.cz</a>

Alternative codes found

RIV/00027162:_____/23:N0000225

Result on the web

<a href="https://www.mdpi.com/2076-2615/13/13/2144" target="_blank" >https://www.mdpi.com/2076-2615/13/13/2144</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani13132144" target="_blank" >10.3390/ani13132144</a>

Result language

angličtina

Original language name

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Original language description

Simple Summary Caseous lymphadenitis is a contagious bacterial disease that affects both domestic and wild animals. It has been studied by scientists since the 19th century. Thanks to new high-throughput RNA sequencing techniques, we can now better understand how the immune system responds to this disease, evaluate individual resistance levels, and identify disease biomarkers. This knowledge can help prevent the spread of incurable diseases, reduce economic losses, and breed farm animals with greater bacterial resistance. In this study, we explore the potential role of immune genes in the fight against disease in ewes from an ordinary farm. The ovine genome reflected host-pathogen interactions by promoting transcriptional changes in innate and acquired immunity in diseased sheep. Furthermore, in exposed sheep, innate immunity increased over the adaptive response. Caseous lymphadenitis (CL) is a chronic contagious disease that affects small ruminants and is characterized by the formation of pyogranulomas in lymph nodes and other organs. However, the pathogenesis of this disease and the response of the host genome to infection are not yet fully understood. This study aimed to investigate the whole blood transcriptome and evaluate differential gene expression during the later stages of CL in naturally infected ewes. The study included diseased, serologically positive (EP), exposed, serologically negative (EN) ewes from the same infected flock and healthy ewes (CN) from a different flock. RNA sequencing was performed using the Illumina NextSeq system, and differential gene expression was estimated using DESeq2 and Edge R approaches. The analysis identified 191 annotated differentially expressed genes (DEGs) in the EP group (102 upregulated and 89 downregulated) and 256 DEGs in the EN group (106 upregulated and 150 downregulated) compared to the CN group. Numerous immunoregulatory interactions between lymphoid and nonlymphoid cells were influenced in both EP and EN ewes. Immune DEGs were preferentially assigned to antigen presentation through the MHC complex, T lymphocyte-mediated immunity, and extracellular matrix interactions. Furthermore, the EP group showed altered regulation of cytokine and chemokine signaling and activation and recombination of B-cell receptors. Conversely, NF-kappa B signaling, apoptosis, and stress response were the main processes influenced in the EN group. In addition, statistically significant enrichment of the essential immune pathways of binding and uptake of ligands by scavenger receptors in EP and p53 signaling in the EN group was found. In conclusion, this study provides new insights into the disease course and host-pathogen interaction in naturally CL-infected sheep by investigating the blood transcriptome.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40203 - Husbandry

Project

<a href="/en/project/QK1910082" target="_blank" >QK1910082: Addressing the problem of the occurrence of bacterial, protozoan and viral zoonotic agents in small ruminant breeds</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animals

ISSN

2076-2615

e-ISSN

—

Volume of the periodical

13

Issue of the periodical within the volume

13

Country of publishing house

CH - SWITZERLAND

Number of pages

20

Pages from-to

2144

UT code for WoS article

001028476800001

EID of the result in the Scopus database

2-s2.0-85165045892