Quantification of cannabinoids and cholesterol in biological materials of different origins via reversed-phase high-performance liquid chromatography with photodiode array detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F25%3A10006221" target="_blank" >RIV/00027014:_____/25:10006221 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S0889157525001309" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0889157525001309</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jfca.2025.107316" target="_blank" >10.1016/j.jfca.2025.107316</a>
Alternative languages
Result language
angličtina
Original language name
Quantification of cannabinoids and cholesterol in biological materials of different origins via reversed-phase high-performance liquid chromatography with photodiode array detection
Original language description
The purpose of our study was to develop original precolumn procedures and C18 high-performance liquid chromatography with a photodiode array detection method for the simultaneous determination of sixteen physiologically important cannabinoids and free cholesterol in plant and animal samples. The precolumn procedure for cannabinoid analysis in nonanimal samples involved two methanol and two n-hexane extractions due to the stronger extraction properties of the methanol solvent. The precolumn procedure in samples of animal origin (homogenized animal tissues, egg yolk of hens and feces), where cholesterol was analyzed together with cannabinoids, consisted of four n-hexane extractions due to the solubility of cannabinoids and cholesterol in n-hexane solvent. Methanol and n-hexane proved to be the appropriate solvents for the extraction of cannabinoids and cholesterol, which occur in assayed biological samples in wide concentration ranges. All the samples were chromatographically analyzed via gradient elution with acetonitrile with formic acid, water with formic acid and methanol. Our chromatographic method, irrespective of the precolumn procedure used, allowed for the selective, precise, accurate and sensitive quantification of cannabinoids and cholesterol in standard solutions and plant and animal samples. The sensitive and selective quantification of cannabinoids is important, especially in food products, because accurate monitoring of psychotropic cannabinoids is particularly necessary.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Food Composition and Analysis
ISSN
0889-1575
e-ISSN
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Volume of the periodical
140
Issue of the periodical within the volume
April
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
1-10
UT code for WoS article
001419607200001
EID of the result in the Scopus database
2-s2.0-85216544372