Isolation and characterization of chitosan - glukan complex from Aspergillus niger biomass

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F01%3A00000033" target="_blank" >RIV/00027022:_____/01:00000033 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Isolation and characterization of chitosan - glukan complex from Aspergillus niger biomass
Original language description
Chitosan and chitin -glucan complex were extracted from Aspergillus niger mycelial wastes of citric acid production.Mycelium was treated with concentratedaqueous NaOH solution (40%) at 130°C for 1-3 h. On applying this procedure, the insoluble material is left as a white powder (15 - 33,5 % on a dry cell basis). For chitosan isolation, this freeze-dried alkali insoluble fraction was treated with diluted HCl solution (2 %) at 95°C for 15-20 h. The yields of the acid soluble chitosan fraction were 5-15 %on a dry cell basis on this condition. IR spectrometry analysis confirmed that the acid soluble material contains chitosan and the acid insoluble remainder consists of chitin and glucan. Results of nitrogen analysis showed a significant prevalence of chitin in the acid insoluble remainder. An original fast and simple method of degree of deacetylation determination was developed using direct UV spectroscopy. Degree of deacetylation of chitosan in the acid soluble fraction is at least 95 %
Czech name
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Czech description
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Project
<a href="/en/project/QE0180" target="_blank" >QE0180: Processing and further industrial utilization of waste mycilium after production of food-grade citric acid.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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