Allergenicity of main birch allergen rBet v1 and high pressure treatment

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F09%3A00000054" target="_blank" >RIV/00027022:_____/09:00000054 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Allergenicity of main birch allergen rBet v1 and high pressure treatment
Original language description
The aim of our study was to determine the influence of high-pressure treatment (HPT) on the structure and allergenicity of recombinant rBet v1, the main birch allergen and birch pollen extract. We treated buffered solutions of rBet v1 and birch pollen extract with high pressure (450-550 MPa) for a period of 10 minutes at temperatures between 30?50 °C. The structural changes in rBet v1 were studied using circular dichroism (CD) spectra. The greatest changes in the rBet v1 structure were found in samplestreated at 450 MPa at 30 °C. Samples treated at 500 and 550 MPa at 30°C (10 minutes) did not show any structural changes. The allergenic reaction to rBet v1 and the birch pollen extract was tested using the Western blot (WB). Pressures of 450 ? 550 MPa (10 minutes) at temperatures of 30, 40 and 50 °C did not change the allergenicity of the rBet v1 protein or the birch pollen extract compared with untreated samples.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Project
<a href="/en/project/2B06139" target="_blank" >2B06139: Inactivation alergens in foods by the high pressure treatment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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