Influence of high pressure treatment on allergenicity of rDau c1 and carrot juice demonstrated by in vitro and in vivo tests

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F09%3A00000057" target="_blank" >RIV/00027022:_____/09:00000057 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Influence of high pressure treatment on allergenicity of rDau c1 and carrot juice demonstrated by in vitro and in vivo tests

Original language description

The aim of our study was to detect the influence of high pressure treatment (HPT) on allergenicity of recombinant allergen rDau c1 and carrot juice using in vitro and in vivo tests. The buffer solution of recombinant main carrot allergen r Dau c1 for basophil activation test (BAT) and Western blot (WB) was used. Dau c1 was pre-treated by pressure 500 MPa for 10 minutes and different temperatures (30, 40, 50°C) and pressure from 400 to 550 MPa for 3 and 10 minutes. Neither the HPT from 400 to 550 MPa for3 and 10 minutes nor the HPT at 500 MPa for 10 minutes and temperature 30, 40 and 50°C had the influence on basophil activation by rDau c1. Serum samples of birch pollen allergic patients reacted in WB with solution of rDau c1 treated by HP 500 MPa for10 minutes at temperature 30, 40 and 50°C. This pressure procedure did not influence the immune reactivity of rDau c1 in WB test. The structural changes of rDau c1 caused by HPT studied by circular dichroismus (CD) spectra were found.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GM - Food industry

OECD FORD branch

—

Project

<a href="/en/project/2B06139" target="_blank" >2B06139: Inactivation alergens in foods by the high pressure treatment</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

High Pressure Research

ISSN

1477-2299

e-ISSN

—

Volume of the periodical

29

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

—

UT code for WoS article

000274675700043

EID of the result in the Scopus database

—