Chitin quantitation (as glucosamine) in food raw materials by spectrophotometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F24%3AN0000013" target="_blank" >RIV/00027022:_____/24:N0000013 - isvavai.cz</a>

Result on the web

<a href="https://methods-x.com/article/S2215-0161(24)00123-7/fulltext" target="_blank" >https://methods-x.com/article/S2215-0161(24)00123-7/fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mex.2024.102669" target="_blank" >10.1016/j.mex.2024.102669</a>

Result language

angličtina

Original language name

Chitin quantitation (as glucosamine) in food raw materials by spectrophotometry

Original language description

Chitin is a water insoluble nitrogen-containing polysaccharide made from N-acetyl-D-glucosamine¨containing β-(1→4)-linkages. In food, chitin is considered as a source of fiber with prebiotic properties to gut microflora. Chitin content varies widely in nature from 1% (yeasts) up to 64% (butterfly cuticles) and is mostly found in filamentous or mushroom forming fungi, insects and crustaceans. This spectrophotometric method is suitable for chitin quantitation (reported as glucosamine) in food raw materials like insects (mealworm larvae, crickets), shrimps, mushrooms and fungi in a research (non-routine) laboratory. To remove interferences, the sample is defatted (Soxhlet) prior to acid hydrolysis in 6 M HCl. The color complex is developed after the addition of Katano’s reagent (a mix of 0.05 mol/L sodium metasilicate, 0.6 mol/L sodium molybdate, 30% dimethyl sulfoxide and 1.42 mol/L acetic acid) at 70 °C for 30 min and measured at 750 nm against blank. A five-point linear calibration (5–100 μg/mL) is used. Limit of detection is 3 μg GLCN/mL. The correlation (R2) with an HPLC method for chitin analysis is at least 0.93. • a reliable alternative to an HPLC method • does not require expensive equipment • deproteination by alkali is not necessary for most matrices - saves about 30% of time

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MethodsX

ISSN

22150161

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

102669

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

1-7

UT code for WoS article

001222725900001

EID of the result in the Scopus database

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