Electrophoretic determination of chitin in insects

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A95082" target="_blank" >RIV/60460709:41210/23:95082 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/23:43928048
Result on the web
<a href="https://doi.org/10.1016/j.chroma.2023.463952" target="_blank" >https://doi.org/10.1016/j.chroma.2023.463952</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2023.463952" target="_blank" >10.1016/j.chroma.2023.463952</a>

Result language
angličtina
Original language name
Electrophoretic determination of chitin in insects
Original language description
An electrophoretic method (on-line coupled capillary isotachophoresis with capillary zone electrophoresis with conductometric detection (cITP-CZE-COND)) for the determination of chitin in insects based on the analysis of glucosamine after acidic hydrolysis of the sample is described. Chitin is deacetylated and hydrolyzed to glucosamine by acidic hydrolysis (6 M sulfuric acid, 110 degrees C, 6 h). Under optimized electrophoresis conditions, glucosamine (GlcN) is separated from other sample components in cationic mode and detected with a conductometer within 15 min. The performance method characteristics of the GlcN assay, i.e. , linearity (0.2-20 mu mol), accuracy (103 +/- 5%), repeatability (1.9%), reproducibility (3.4%), limits of detection (0.06 mu mol/L) and quantification (0.2 mu mol/L), were evaluated. On a series of 28 insect samples, it was proven that cITP-CZE-COND provides results of chitin content in insects comparable to the literature data. The important features of the developed cITP-CZE-COND method are easy sample treatment, high sensitivity and selectivity, and low running costs. It is clear from the above that the cITP-CZE-COND method is suitable for analysis of insect samples for chitin content. (c) 2023 Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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