Interlaboratory study on real-time PCR detection and quantification of European anglerfish (L. budegassa and L. piscatorius) and seabream (Spondyliosoma cantharus) parvalbumin gene

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F24%3AN0000025" target="_blank" >RIV/00027022:_____/24:N0000025 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s00217-024-04578-w" target="_blank" >https://link.springer.com/article/10.1007/s00217-024-04578-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00217-024-04578-w" target="_blank" >10.1007/s00217-024-04578-w</a>

Result language

angličtina

Original language name

Interlaboratory study on real-time PCR detection and quantification of European anglerfish (L. budegassa and L. piscatorius) and seabream (Spondyliosoma cantharus) parvalbumin gene

Original language description

This study presents a large-scale interlaboratory comparison (ILC) aimed at detecting and quantifying DNA from two European anglerfish (Lophius budegassa, Lophius piscatorius), pike (Esox lucius) and sea bream (Spondyliosoma cantharus) using real-time qPCR. To detect amplification of the parvalbumin genetic marker, single and multiplex qPCR assays using EvaGreen® dye or TaqMan™ probes were used. Genomic DNA isolated from target fish species and an advanced DNA calibrator, gBlocks® gene fragments, were used as standards. The DNA of anglerfish, pike and sea bream as well as their mixtures were analysed together with 14 other non-target fish species. All target fish samples were correctly identified by the participating laboratories. Qualitative assessment of anglerfish and seabream DNA showed an accuracy rate of 100%, while pike DNA achieved a match rate of 99%. Validation of quantitative protocols in four different laboratories consistently achieved z-scores below 2, indicating satisfactory performance and confirming the high degree of similarity of laboratory results. Furthermore, high accuracy and efficiency were demonstrated for the quantification of anglerfish and seabream DNA by triplex qPCR using TaqMan™ probes. Regarding the selected gene marker, the major fish allergenic protein parvalbumin enables indirect detection and quantification of the allergen in the sample. Therefore, the use of proposed protocols can significantly contribute to protecting the health of consumers and to controlling the food market.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10700 - Other natural sciences

Project

<a href="/en/project/QK1910231" target="_blank" >QK1910231: New approaches for the proof of fish meat adulteration using genomic DNA</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Food Research and Technology

ISSN

1438-2377

e-ISSN

—

Volume of the periodical

250

Issue of the periodical within the volume

11

Country of publishing house

DE - GERMANY

Number of pages

15

Pages from-to

2821 - 2835

UT code for WoS article

001228525600001

EID of the result in the Scopus database

2-s2.0-85193715067