Parvalbumin Gene: A Valuable Marker for Pike Authentication and Allergen Risk Assessment

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F24%3AN0000027" target="_blank" >RIV/00027022:_____/24:N0000027 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/24:10481832 RIV/60461373:22330/24:43930334
Result on the web
<a href="https://pubs.acs.org/doi/full/10.1021/acs.jafc.4c01410" target="_blank" >https://pubs.acs.org/doi/full/10.1021/acs.jafc.4c01410</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jafc.4c01410" target="_blank" >10.1021/acs.jafc.4c01410</a>

Result language
angličtina
Original language name
Parvalbumin Gene: A Valuable Marker for Pike Authentication and Allergen Risk Assessment
Original language description
Fish from the pike (Esox) genus are valued in gastronomy for their superior meat quality. However, they can cause allergic reactions in sensitive consumers. This work aimed to fill the gap in the detection of pike allergens using molecular–biological techniques. New, fast, and accurate loop-mediated isothermal amplification (LAMP) and real-time PCR (qPCR) assays were designed to detect pike DNA using the parvalbumin gene as a marker. LAMP was assessed by electrophoresis, SYBR green optical detection, and real-time fluorescence detection. The latter was the most sensitive, detecting as little as 0.78 ng of pike DNA; the qPCR detection limit was 0.1 ng. The LAMP analysis took 20–70 min, which is significantly faster than qPCR. The study provides reliable detection and quantification of the parvalbumin gene in both fresh and processed samples and further highlights the versatility of the use of the parvalbumin gene for the authentication of food products and consumer protection via refined allergen risk assessment that is independent of the type of tissue or food processing method used.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10700 - Other natural sciences

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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