In vitro root formation in Pulsatilla vernalis (L.) MILL.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027073%3A_____%2F12%3A%230001458" target="_blank" >RIV/00027073:_____/12:#0001458 - isvavai.cz</a>

Result on the web

<a href="http://www.journal-pop.org/2012_12_2_96-101.html" target="_blank" >http://www.journal-pop.org/2012_12_2_96-101.html</a>

DOI - Digital Object Identifier

—

Result language

čeština

Original language name

In vitro root formation in Pulsatilla vernalis (L.) MILL.

Original language description

An efficient in vitro rooting system was developed for the endangered and difficult-to-root spring pasque flower (Pulsatilla vernalis (L.) Mill). Rhizogenesis of shoots was observed on half-strength woody plant agar medium only with high concentration ofindole-3-butyric acid (IBA). Optimal rooting was observed for incised shoots grown on half-strength woody plant perlite medium with 20 mg l-1 IBA for 3 weeks and then on an expressive medium. Under these conditions 84.7% of the shoots rooted. The incision of shoots (on the base of shoot) had significantly beneficial effects on root induction. Application of auxin during in vitro rhizogenesis affected subsequent acclimatization of plantlets. The highest plantlet survival rate (90.5%) was observed in plants which were dipped in IBA and then exposured to the induction medium for 3 weeks during in vitro phase. The plants were replanted and transferred to a shady hotbed, and were ready for planting in the next year.

Czech name

In vitro root formation in Pulsatilla vernalis (L.) MILL.

Czech description

An efficient in vitro rooting system was developed for the endangered and difficult-to-root spring pasque flower (Pulsatilla vernalis (L.) Mill). Rhizogenesis of shoots was observed on half-strength woody plant agar medium only with high concentration ofindole-3-butyric acid (IBA). Optimal rooting was observed for incised shoots grown on half-strength woody plant perlite medium with 20 mg l-1 IBA for 3 weeks and then on an expressive medium. Under these conditions 84.7% of the shoots rooted. The incision of shoots (on the base of shoot) had significantly beneficial effects on root induction. Application of auxin during in vitro rhizogenesis affected subsequent acclimatization of plantlets. The highest plantlet survival rate (90.5%) was observed in plants which were dipped in IBA and then exposured to the induction medium for 3 weeks during in vitro phase. The plants were replanted and transferred to a shady hotbed, and were ready for planting in the next year.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Propagation of Ornamental Plants

ISSN

1311-9109

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

2

Country of publishing house

BG - BULGARIA

Number of pages

6

Pages from-to

96-101

UT code for WoS article

000306541700003

EID of the result in the Scopus database

—