Organogenesis of pedunculate oak (Quercus robur L.)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41410%2F05%3A10864" target="_blank" >RIV/60460709:41410/05:10864 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Reprodukce dubu letního (Quercus robur L.) pomocí organogeneze

Original language description

We tested a method of in vitro micropropagation of Q. robur L. Primary explants were axillary shoots. Multiple shoots were induced on Woody plant medium (WPM) and MS medium supplemented with 6-benzylaminopurine (BAP 2.25 - 22.5 &#956;M). The highest shoot proliferation rate was achieved on WP medium supplemented with BAP (13.5 &#956;M). Rooting of regenerated shoots involved a two-step procedure. During the first phase microshoots were treated with varying concentration of indole-3-butyric acid (IBA 0-80 &#956;M) for 12 hr, 24 hr and 48 hr. In the second phase microshoots were transferred to a plant growth regulator-free half-strength WP medium. The best results of rooting were achieved when microshoots were treated with 60 &#956;M IBA for 24 hr and then placed on PGR-free half-strength WP medium.

Czech name

Reprodukce dubu letního (Quercus robur L.) pomocí organogeneze

Czech description

We tested a method of in vitro micropropagation of Q. robur L. Primary explants were axillary shoots. Multiple shoots were induced on Woody plant medium (WPM) and MS medium supplemented with 6-benzylaminopurine (BAP 2.25 - 22.5 &#956;M). The highest shoot proliferation rate was achieved on WP medium supplemented with BAP (13.5 &#956;M). Rooting of regenerated shoots involved a two-step procedure. During the first phase microshoots were treated with varying concentration of indole-3-butyric acid (IBA 0-80 &#956;M) for 12 hr, 24 hr and 48 hr. In the second phase microshoots were transferred to a plant growth regulator-free half-strength WP medium. The best results of rooting were achieved when microshoots were treated with 60 &#956;M IBA for 24 hr and then placed on PGR-free half-strength WP medium.

Type

D - Article in proceedings

CEP classification

GK - Forestry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník z konference COYOUS 2004

ISBN

80-213-1318-8

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

0-0

Publisher name

ČZU Fakulta lesnická a environmentální

Place of publication

Praha

Event location

Praha

Event date

Dec 3, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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