Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using X- and Y- bovine (Bos taurus L.) painting probes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F04%3A00012679" target="_blank" >RIV/00027162:_____/04:00012679 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using X- and Y- bovine (Bos taurus L.) painting probes

Original language description

River buffalo, sheep and goat spermatozoa were cross-hybridized for double color FISH with bovine X and Y-chromosome painting probes, prepared by DOP-PCR of laser-microdissected-catapulted chromosomes, to investigate the possibility of using bovine probes for sexing sperm of other members of the family Bovidae. FISH analysis of more than twelve thousands sperm revealed high level of X-Y signals on all species investigated. Significant interspecific differences (P&lt;0.01) were detected in the frequencyof aberrant spermatozoa (aneuploid and diploid) between goat (0.393%) and sheep (0.033%) followed by river buffalo (0.224%) and cattle (0.096%). The present study demonstrated that it is possible to use bovine X-Y painting probes for sexing and analyzingsperm of other species of the family, thus facilitating future studies on the incidence of chromosome abnormalities in sperm as well as on sex predetermination of embryos for the livestock industry.

Czech name

Sexování spermií búvola vodního (Bubalus bubalis L.), ovce (Ovis aries L.), kozy (Capra hircus L.) a skotu (Bos taurus L.) dvoubarevnou s použitím X a Y malovacích sond

Czech description

Spermie buvola vodního, ovce a kozy byly hybridizovány dvoubarevnou FISH s boviními malovacími sondami připravenými pomocí mikrodisekce a DOP-PCR. Signifikantní mezidruhové diference (P&lt;0.01) byly zjištěny ve frekvenci aberantních spermií (aneuploidiea diploidie) mezi kozou (0.393%), ovcí (0.033%), buvolem vodním (0.224%) a skotem (0.096%). Bylo demonstrováno, že boviní malovací sondy pro chromozom X a Y lze použít pro sexování a analýzu spermií ostatních druhů čeledi Bovidae.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA523%2F01%2F0291" target="_blank" >GA523/01/0291: Development of painting DNA probes by laser microdissection of bovine chromosomes and their use in the diagnostics of chromosomal aberrations</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Reproduction and Development

ISSN

1040-452X

e-ISSN

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Volume of the periodical

67

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

108-115

UT code for WoS article

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EID of the result in the Scopus database

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