Isolation and partial characterization of a novel porcine astrovirus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F06%3A%230000122" target="_blank" >RIV/00027162:_____/06:#0000122 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isolation and partial characterization of a novel porcine astrovirus

Original language description

Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a dirrhoeal specimen. Furthemore, a cytopathic virus was isolated and propagated in an established porcine kidney cell line, PK-15. Reverse transcription and PCR performed with astrovirus-specific primers amplified a product with the expected size. Sequencing of the PCR product revealed that the virus observed by electron microscopy and propagated in the porcine cell line is an astrovirus, showing 86% identity at the nucleotide level with the only known porcine astrovirus,PAstV. Phylogenetic analysis clustered the novel isolate,Sb4685, together with PAstV in a broad clade comprising mammalian astroviruses.

Czech name

Izolace a částečná charakterizace nového astroviru prasat

Czech description

Je popsána astrovirová infekce prasat jako příčina průjmu u selat. Virus byl prokázán ve vzorku trusu z průjmujícího selete a izolován na buněčné línii PK-15. Pro průkaz astroviru byla použita metoda RT-PCR a sekvenování PCR produktu. Sekvenování prokázalo 86% identitu nukleotidů s jediným dosud známým astrovirem prasat, PAstV. Byla také provedena fylogenetická analýza nového izolátu, Sb4685. Jedná se o prioritní izolaci astroviru prasat v ČR a druhou izolaci ve světě.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

<a href="/en/project/QF4051" target="_blank" >QF4051: The use of biotechnological procedures in the diagnosis and immunoprophylaxis of viral gastroenteritides of swine</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

2-4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

2-4

UT code for WoS article

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EID of the result in the Scopus database

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