The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000551" target="_blank" >RIV/00027162:_____/09:#0000551 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The mycotoxin deoxynivalenol promotes uptake of Salmonella Typhimurium in porcine macrophages, associated with ERK 1/2 induced cytoskeleton reorganization

Original language description

This study aimed at examining the interaction between mycotoxin deoxynivalenol (DON) and Salmonella Typhimurium at the level of the porcine innate immune system, represented by macrophages. First, we assessed the direct cytotoxic effect of DON on porcinemacrophages. Incubation with 0.25 ug/ml of DON or higher resulted in a significant cytotoxic effect after 24 h of incubation. Secondly, the direct toxic effect of DON on the growth and on the expression of Salmonella pathogenicity island 1 (SPI-1) and SPI-2 virulence genes of S. Typhimurium was determined. At low non-cytotoxic concentrations, DON was of no effect on growth or virulence gene expression of S. Typhimurium. However, when the invasion and intracellular survival of S. Typhimurium in macrophages preexposed to 0.025 ug/ml of DON was examined, DON significantly promoted the uptake of S. Typhimurium into macrophages which coincided with F-actin reorganization due to the activation of ERK1/2.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Research

ISSN

0928-4249

e-ISSN

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Volume of the periodical

40

Issue of the periodical within the volume

6

Country of publishing house

FR - FRANCE

Number of pages

12

Pages from-to

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UT code for WoS article

000272039500013

EID of the result in the Scopus database

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