Immunohistochemical Detection of Wheat Protein in Model Samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F10%3A%230000698" target="_blank" >RIV/00027162:_____/10:#0000698 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16270/10:00002991
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Immunohistochemical Detection of Wheat Protein in Model Samples
Original language description
The study focused on optimization of immunohistochemical examination for gluten content detection in model samples (pork meat with wheat semi-smooth flour, pork meat with wheat protein edible vital). The best results were achieved by immunohistochemicalmethod based on ABC (avidin - biotin complex) method utilizing polyclonal antibodies diluted 1:1000. Ther results demonstrate that for pure wheat protein detection utilization immunohistochemical detection, which can detect already 0.1 % of added wheat protein, is more advantageous, while the commonly used ELISA method reliably probates this additive approximately from 0.4 % upwards.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Project
<a href="/en/project/1B53004" target="_blank" >1B53004: Qualitative and quantitative diagnostics of particles of plant and animal origin in foodstuffs using histochemical or immunohistochemical methods.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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