Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000731" target="_blank" >RIV/00027162:_____/11:#0000731 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparing electron microscopy and a competitive blocking ELISA in the detection of rotaviruses in porcine faeces

Original language description

Analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (EM) and a competitive blocking (CB)-ELISA based on monoclonal antibodies to the VP6 protein of group A rotavirus. Rotavirus was detected by EM and/or CB-ELISAin 111 (23.3%) samples. Of these, all groups of rotavirus were identified in 83 (74.8%) samples by EM (EM plus), while group A rotavirus was identified in 90 (81.1%) samples by CB-ELISA (ELISA plus). However, only 62 (55.9%) of samples were positive using both detection methods. The finding of 28 (25.2%) EM-/CB-ELISA plus samples illustrated the high sensitivity of the CB-ELISA method. On PCR analysis, groups B and C rotavirus was found in 3 and 16 0119 EMplus/CB-ELISA- samples, respectively. Althoughthe study illustrates the high sensitivity of a CB-ELISA in rotavirus detection, the findings highlight the need to use a range of diagnostic methods in detecting these viruses in clinical samples. (C) 2009 Elsevier Ltd. All rights reserv

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/QF4051" target="_blank" >QF4051: The use of biotechnological procedures in the diagnosis and immunoprophylaxis of viral gastroenteritides of swine</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Journal

ISSN

1090-0233

e-ISSN

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Volume of the periodical

187

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

3

Pages from-to

279-281

UT code for WoS article

000288520000029

EID of the result in the Scopus database

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