Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000743" target="_blank" >RIV/00027162:_____/11:#0000743 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR

Original language description

This study focused on the development of a DNA isolation procedure for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) in faeces by IS900 and F57 quantitative real time PCR (qPCR) and their comparison with culture. The recovery of MAPDNA ranged from 29.1 to 102.4% of the input amount of MAP with median 37.9%. The limit of detection was determined to be 1.03 x 10(4) for F57 qPCR and 6.87 x 10(2) MAP cells per gram of faeces for IS900 qPCR, respectively. The developed technique for DNAisolation was coupled with 15900 qPCR and compared to traditional MAP culture using a cohort of 1906 faecal samples examined from 12 dairy cattle farms in our laboratory. From those 1906 original faecal samples. 875 were positive by IS900 qPCR and 169 by culture. This data facilitated development of a predictive model capable of estimating the probability of being culture positive by estimating the absolute number of MAP per gram of faeces as determined IS900 qPCR without performing the

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

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Volume of the periodical

149

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

133-138

UT code for WoS article

000289031600016

EID of the result in the Scopus database

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