Cell-mediated immune response in swine infected with Mycobacterium avium subsp. avium

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000744" target="_blank" >RIV/00027162:_____/11:#0000744 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Cell-mediated immune response in swine infected with Mycobacterium avium subsp. avium
Original language description
We analysed porcine antigen-specific immunity six months after experimental infection with Mycobacterium avium subsp. avium (MAA) by measuring IFN-gamma production (ELISA, qRT-PCR and flow cytometry) and lymphocyte transformation test after in vitro re-stimulation with the MAA-derived antigen. In MAA-infected animals, IFN-gamma was increased at both mRNA and protein levels in response to the specific MAA antigen in the blood, spleen and mesenteric lymph nodes. Similarly, a positive antigen-driven response was detected by proliferation assay. Using flow cytometry, we found double-positive CD4+CD8+ lymphocytes as the major T lymphocyte subset producing IFN-gamma after specific re-stimulation. In conclusion, antigen-specific immune response in swine is retained for at least six months after infection with MAA. Furthermore, we identified the presence of antigen-specific double-positive CD4+CD8+ cells in MAA-infected pigs.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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