Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000761" target="_blank" >RIV/00027162:_____/11:#0000761 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle Persistence of Mycobacterium avium subsp paratuberculosis at a Farm-Scale Biogas Plant Supplied with Manure from Paratuberculosis-Affected Dairy Cattle
Original language description
Products from all steps of anaerobic digestion at a farm-scale biogas plant supplied with manure from paratuberculosis-affected cattle were examined for the presence of Mycobacterium avium subsp. paratuberculosis (MAP). Viable MAP cells were detected using culture in fermentors for up to 2 months; the presence of MAP DNA was demonstrated in all anaerobic fermentors and digestate up to 16 months after initiation of work at a biogas plant, using IS900 real time PCR (qPCR). According to these results, a fermentation process that extended beyond 2 months removed all viable MAP cells and therefore rendered its product MAP free. MAP DNA was found during all the examined periods, which could be explained by either residual DNA being released from dead cells or by the presence of viable cells whose amount was under the limit of cultivability. As the latter hypothesis cannot be excluded, the safety of the final products of digestion used for fertilization or animal bedding cannot be defined.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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