Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000802" target="_blank" >RIV/00027162:_____/11:#0000802 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Association of attenuated mutants of Salmonella enterica serovar Enteritidis with porcine peripheral blood leukocytes

Original language description

We were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis (with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and delta SPI1-5 mutant) with subpopulations of porcine white blood cells (WBC). Using flow cytometry, we did not observe any difference in the interactions of the wild-type S. Enteritidis, aroA and phoP mutants with WBC. Delta SPI1-5 and fliC mutants had a minor defect in their association with granulocytes and monocytes. All three rfa mutants associated with granulocytes, monocytes and B-lymphocytes more than the wildtype S. Enteritidis did. Electron microscopy showed that the Salmonella-containing vacuole in the WBC infected with the rfa mutants, unlike all other strains, did not developinto a spacious phagosome. Intact lipopolysaccharide, but not the type III secretion system encoded by SPI-1, SPI-2 or the flagellar operon, is important for the initial interaction of S. Enteritidis with porcine leukocytes.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEMS MICROBIOLOGY LETTERS

ISSN

0378-1097

e-ISSN

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Volume of the periodical

321

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

37-42

UT code for WoS article

000292337200006

EID of the result in the Scopus database

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