Genotoxicity of 7H-dibenzo[c,g]carbazole and Its Tissue-Specific Derivatives in Human Hepatoma HepG2 Cells Is Related to CYP1A1/1A2 Expression
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000816" target="_blank" >RIV/00027162:_____/11:#0000816 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/11:00368653
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Genotoxicity of 7H-dibenzo[c,g]carbazole and Its Tissue-Specific Derivatives in Human Hepatoma HepG2 Cells Is Related to CYP1A1/1A2 Expression
Original language description
The goal of this study was to investigate the genotoxicity of 7H-dibenzo[c,g]carbazole (DBC), a ubiquitous environmental pollutant, and its methyl derivatives, 5,9-dimethylDBC (DiMeDBC), a strict hepatocarcinogen, and N-methylDBC (N-MeDBC), a specific sarcomagen in human hepatoma HepG2 cells, and to infer potential mechanisms underlying the biological activity of particular carcinogen. All dibenzocarbazoles, regardless the tissue specificity, induced significant DNA strand break levels and micronuclei in HepG2 cells; though a mitotic spindle dysfunction rather than a chromosome breakage was implicated in N-MeDBC-mediated micronucleus formation. While DBC and N-MeDBC produced stable DNA adducts followed with p53 protein phosphorylation at Ser-15, DiMeDBC failed. A significant increase in DNA strand breaks following incubation of exposed cells with a repair-specific endonuclease (Fpg protein) suggested that either oxidative DNA damage or unstable DNA-adducts might underlie DiMeDBC genoto
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DN - Environmental impact on health
OECD FORD branch
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Result continuities
Project
<a href="/en/project/2B08005" target="_blank" >2B08005: New approaches to study the toxicity of the air pollution and their contribution to assess limits for selected pollutants.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental and Molecular Mutagenesis
ISSN
0893-6692
e-ISSN
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Volume of the periodical
52
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
636-645
UT code for WoS article
000296861100005
EID of the result in the Scopus database
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