Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F12%3A%230000882" target="_blank" >RIV/00027162:_____/12:#0000882 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.tvjl.2012.05.002" target="_blank" >http://dx.doi.org/10.1016/j.tvjl.2012.05.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tvjl.2012.05.002" target="_blank" >10.1016/j.tvjl.2012.05.002</a>

Result language
angličtina
Original language name
Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture
Original language description
Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, has a lipid-rich cell wall which facilitates its survival and persistence in the environment. This property of the organism is exploited when it is cultured as decontaminating agents and antibiotics are used to suppress the growth of contaminating microflora, but such treatments can also negatively affect the isolation of MAP itself. The objective of this study was to assess the effect of the 'VAN' antibiotics (vancomycin, amphotericin B and nalidixic acid) on the viability of MAP using a propidium monoazide real time quantitative PCR (PMA qPCR) and culture. Long-term (5 week) treatment with VAN antibiotics resulted in a larger decrease in bacterialnumbers compared to short-term (3 day) exposure. The PMA qPCR assay indicated that 50 mu g/mL of vancomycin, 50 mu g/mL of nalidixic acid, and 200 mu g/mL of amphotericin B were 'threshold' concentrations, respectively, above which the d
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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