Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001128" target="_blank" >RIV/00027162:_____/14:#0001128 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.tvjl.2014.05.032" target="_blank" >http://dx.doi.org/10.1016/j.tvjl.2014.05.032</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tvjl.2014.05.032" target="_blank" >10.1016/j.tvjl.2014.05.032</a>

Result language
angličtina
Original language name
Repeated cycles of chemical and physical disinfection and their influence on Mycobacterium avium subsp paratuberculosis viability measured by propidium monoazide F57 quantitative real time PCR
Original language description
Mycobacterium avium subsp. paratuberculosis (MAP) has a high degree of resistance to chemical and physical procedures frequently used for the elimination of other bacteria. Recently, a method for the determination of viability by exposure of MAP to propidium monoazide (PMA) and subsequent real time quantitative PCR (qPCR) was established and found to be comparable with culture. The aim of this study was to apply the PMA qPCR method to determine the impact of increasing concentration or time and repeatedcycles of the application of selected disinfectants on MAP viability. Different MAP isolates responded to the same type of stress in different ways. The laboratory strain CAPM 6381 had the highest tolerance, while the 8819 low-passage field isolate wasthe most sensitive. Ultraviolet exposure caused only a partial reduction in MAP viability; all MAP isolates were relatively resistant to chlorine. Only the application of peracetic acid led to the total elimination of MAP. Repeated applic
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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