Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F12%3A%230000955" target="_blank" >RIV/00027162:_____/12:#0000955 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/12:00068283

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Boar sperm head membrane damage during cryopreservation evaluated by electron microscopy

Original language description

As electron microscopy can reveal much smaller changes in plasma membrane integrity than optical methods, it appears to be a useful tool for evaluating semen quality or the damage inflicted on spermatozoa during preservation. By evaluating membrane integrity of the sperm head in 16 boars by transmission electron microscopy (TEM), it was found that the plasma membrane is very sensitive and tends to break in the acrosomal area but not in the rest of the sperm head. The earliest signs of injury to spermatozoa in the acrosomal part of the sperm head were observed in the plasma membrane that became swollen, broken or lost. The next damaged part of spermatozoa which is less sensitive than the plasma membrane is the acrosome and its external membrane. The freezing process caused a substantial increase in the degree of cell damage manifested as disturbed or missing plasma membrane, acrosomal reaction-like changes and unevenly distributed or lost acrosomal contents.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

<a href="/en/project/QI111A166" target="_blank" >QI111A166: Biotechnological processes in reproduction and pig breeding as a tool of the economic growth and competitive advantage.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

58-61

UT code for WoS article

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EID of the result in the Scopus database

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